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KMID : 1132720200180030032
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Genomics & Informatics
2020 Volume.18 No. 3 p.32 ~ p.32
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Sequencing and annotation of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba
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Islam Mohammad Nazrul
Sultana Shirin
Alam Md. Jobaidul
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Abstract
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The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).
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KEYWORD
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complete mitogenome, Illumina sequencing, mitochondrial DNA, reba carp, threatened species
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